PO.TB02.01 · 肿瘤生物学
优化的铁碳纳米颗粒在磁热疗中表现出优越的体内抗肿瘤疗效
Optimized iron-carbon nanoparticles demonstrate superior; in vivo antitumor efficacy in magnetic hyperthermia treatment
作者与单位 Authors & Affiliations
摘要 Abstract
中文摘要
本研究旨在开发和评估为基于磁热疗的癌症治疗而合成的铁碳（Fe/C）纳米颗粒，作为我们此前在相同细胞系上发表的体外工作的延续。采用共沉淀法并优化前体比例制备 Fe/C 纳米颗粒，以增强磁性能。结构和磁学表征揭示了性能的改善，饱和磁化强度为 148.6 emu/g。在 MCF-7 乳腺癌细胞中的体外细胞毒性检测显示，在较低的纳米颗粒剂量下即出现显著的细胞死亡，证实了有效的热疗活性。使用 DU145 前列腺癌和 MCF-7 乳腺癌异种移植模型进行了体内实验，在瘤内注射 50 μg/mL 和 100 μg/mL 的 SPINs 后施加交变磁场。50 μg/mL 剂量导致肿瘤逐渐缩小，而 100 μg/mL 剂量则诱导了快速而均匀的缩小，在多个病例中接近完全消退。热成像验证了肿瘤内的温度升高。这些发现表明，Fe/C SPINs 是用于癌症治疗中靶向、微创磁热疗单药治疗的有前景的药剂。
查看英文原文 English abstract
This study aims to develop and evaluate iron-carbon (Fe/C) nanoparticles synthesized for magnetic hyperthermia-based cancer therapy, as a continuation of our previously published in vitro work on the same cell lines. The Fe/C nanoparticles were prepared using a co-precipitation method with optimized precursor ratios to enhance magnetic performance. Structural and magnetic characterization revealed improved properties, with a saturation magnetization of 148.6 emu/g. In vitro cytotoxicity assays in MCF-7 breast cancer cells showed significant cell death at lower nanoparticle doses, confirming effective hyperthermic activity. In vivo experiments were performed using DU145 prostate and MCF-7 breast cancer xenograft models, where intratumoral injections of SPINs at 50 µg/mL and 100 µg/mL were followed by alternating magnetic field exposure. The 50 µg/mL dose led to gradual tumor reduction, while the 100 µg/mL dose induced rapid and uniform shrinkage, with near-complete regression in several cases. Thermal imaging verified temperature increase within the tumors. These findings indicate that Fe/C SPINs are promising agents for targeted and minimally invasive magnetic hyperthermia monotherapy in cancer treatment.
利益披露 Disclosure
M. Elabbasi, None..
S. Cooper, None.