PO.CL01.23 · 临床研究
用于癌症患者循环肿瘤细胞簇的无离心微流控分离平台的方法学与临床验证
Methodology and clinical validation of a centrifugation-free microfluidic isolation platform for circulating tumor cell clusters in cancer patients
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摘要 Abstract
中文摘要
背景：循环肿瘤细胞（CTC）簇是外周血中发现的肿瘤细胞聚集体，通常与不良临床结局相关。然而，目前能够在无需预先血液处理的情况下分离、计数或培养这些细胞簇的技术仍然有限。
方法：我们开发了一种微流控单细胞挑选系统，能够在无需样本制备或离心的情况下分离细胞，从而保留其天然形态和活力。与常规离心或基于磁珠的富集方法（往往会损伤脆弱的细胞簇）不同，该平台将实时荧光激活液滴提取与坐标引导挑选相结合，选择性地回收单细胞和完整细胞簇，用于下游多组学研究。
结果：该系统展现出93%的线性回收效率（R²=0.9985），与基于磁珠的技术相比，维持了更高水平的细胞活力和代谢功能。在临床检测中，该系统可根据CTC和CTC簇计数区分癌症患者与健康个体，凸显了其在液体活检应用和精准肿瘤学中的价值。
结论：这项概念验证工作表明，一种温和、无需预处理的微流控策略能够有效分离和表征单细胞及完整细胞簇，为理解转移生物学提供了见解，并增强了临床诊断工具的潜力。
查看英文原文 English abstract
BACKGROUND: Circulating tumor cell (CTC) clusters are aggregates of tumor cells found in the peripheral blood and are typically linked to unfavorable clinical outcomes. However, current technologies capable of isolating, counting, or culturing these clusters without prior blood processing remain limited.
METHODS: We developed a microfluidic single-cell-picking system that enables isolation of cells without sample preparation or centrifugation, thereby preserving their native morphology and viability. Unlike conventional centrifugation or bead-based enrichment-which often compromises fragile clusters-this platform combines real-time fluorescence-activated droplet extraction with coordinate-guided picking to selectively retrieve single cells and intact clusters for downstream multi-omics investigation.
RESULTS: The system demonstrated a linear recovery efficiency of 93% (R² = 0.9985) and sustained higher levels of cell viability and metabolic function compared with magnetic bead-based techniques. In clinical testing, it distinguished cancer patients from healthy individuals based on CTC and CTC-cluster counts, underscoring its value for liquid biopsy applications and precision oncology.
CONCLUSION: This proof-of-concept work indicates that a gentle, preprocessing-free microfluidic strategy can effectively isolate and characterize both single cells and intact clusters, offering insights into metastatic biology and enhancing the potential of clinical diagnostic tools.
利益披露 Disclosure
J. Hsieh, None..
P. Chang, None..
T. Chiu, None.