PO.CL07.02 · 临床研究
肿瘤基质细胞上的Periostin可能与胃癌患者的恶性进展相关
Periostin on tumor stromal cells might be associated with the malignant progression of patients with gastric cancer
作者与单位 Authors & Affiliations
摘要 Abstract
中文摘要
Periostin（骨膜蛋白）是基质细胞蛋白之一。据报道，肿瘤微环境中的periostin可能通过与癌细胞上表达的整合素相互作用来调控FAK信号通路，从而刺激增殖和侵袭。在胃癌（GC）中，periostin已被报道在肿瘤基质中过表达，然而，periostin的作用及其表达细胞类型仍不明确。于是，在本研究中，我们使用689例GC样本，通过免疫组化研究检验了periostin对GC临床病理特征的意义。此外，使用4株GC细胞系，通过增殖实验和侵袭实验检验periostin对GC细胞增殖和侵袭活性的影响。periostin的免疫组化研究证实，periostin的表达常见于肿瘤内的基质成纤维细胞上。肿瘤基质中periostin的高表达与更差的生存率（p < 0.001，log-rank）、更深的T浸润（p < 0.001）、更频繁的淋巴结转移（p < 0.001）、远处转移（p = 0.021）、更高的复发率（p < 0.001）以及更高的分期（p < 0.001）相关。使用GC细胞系的体外实验表明，periostin显著促进GC细胞的侵袭和迁移能力，但不影响其增殖活性。总之，主要表达于肿瘤基质成纤维细胞上的periostin可能与GC的恶性进展相关。
查看英文原文 English abstract
Periostin is one of matrix cellular proteins. It has been reported that the periostin in the tumor microenvironment might regulate the FAK signaling via the interaction with integrins expressed on cancer cells, resulting in the stimulation of proliferation and invasion. In gastric cancer (GC), the periostin has reported to be overexpressed in the tumor stroma, however, the role of periostin and the types of cells remains to be unclear. Then, in this study we examined the significance of periostin on the clinico-pathologic features of GC by immunohistochemical study, using 689 GC samples. Also, 4 GC cell lines were used to examine the effect of periostin on the proliferation and the invasion activity of GC cells by proliferation assay and invasion assay.The immunohistochemical study of periostin confirmed that the expression of periostin was frequently found on stromal fibroblasts in the tumor. High expression of periostin in the tumor stroma is associated with worse survival rates (p < 0.001, log-rank), deeper T invasion (p < 0.001), frequent lymph node metastasis (p < 0.001), distant metastasis (p = 0.021), higher recurrence rates (p < 0.001), and higher stage (p < 0.001). I n vitro assays using GC cell lines demonstrated that periostin significantly promoted the invasion and migration ability of GC cells, but did not affect the proliferation activity.In conclusion, the periostin, which is mainly expressed on the tumor stromal fibroblasts, might be associated with the malignant progression of GC.
利益披露 Disclosure
C. Fan, None..
H. Nishikubo, None.