PO.TB10.14 · 肿瘤生物学

Patients with high body mass index share tumor microbial profiles with CpG island methylator phenotype-high or early-onset colorectal cancers

海报缩略图：Patients with high body mass index share tumor microbial profiles with CpG island methylator phenotype-high or early-onset colorectal cancers

编号 4890 展板 6 时间 4/21 09:00–12:00 区域 Section 29 主讲 KuoHsing Chen, MD;PhD

分会场 Microbiome-Tumor-Immune Crosstalk

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作者与单位

KuoHsing Chen¹, Jyh-Shiuan Hsu², Yu-Li Su³, Shih-Chiang Lin⁴, Been-Ren Lin⁵, Kai-Lung Tsai⁶, Li-Ming Tseng⁷, Yi-Hsin Liang⁸, Jia-Huei Tsai⁹, Chien-Chen Tsai¹⁰, Ting-Ting Liu¹¹, Mong-Hsun Tsai¹², Kun-Huei Yeh¹³

¹Medical Oncology, National Taiwan University Cancer Center (NTUCC), Taipei City, Taiwan,²Institute of Biotechnology, National Taiwan University, Taipei, Taiwan., Taipei City, Taiwan,³Division of Hematology Oncology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan,⁴Oncology and Hematology, Far Eastern Memorial Hospital, New Taipei City, Taiwan,⁵Surgery, National Taiwan University Cancer Center, Taipei, Taiwan,⁶Division of Colon & Rectal Surgery, Kaohsiung Chang Gung Memorial Hospital,, Kaohsiung, Taiwan,⁷Division of Colorectal Surgery, Far Eastern Memorial Hospital, New Taipei City, Taiwan,⁸National Taiwan University College of Medicine, Taipei, Taiwan,⁹Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan,¹⁰Department of Anatomical pathology, Far Eastern Memorial Hospital, New Taipei City, Taiwan,¹¹Department of Anatomic Pathology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan,¹²Institute of Biotechnology, National Taiwan University, Taipei, Taiwan,¹³Dept. of Oncology, National Taiwan University Hospital, Taipei, Taiwan

摘要 Abstract

Background Tumor microbiota plays a significant role in colorectal cancer (CRC) by influencing tumor development and progression. Our previous study demonstrated a significant association between high body mass index (high BMI, BMI ≥ 27.5 kg/m 2 ) and CpG island methylator phenotype-high (CIMP-high) in early-onset CRC (EOCRC, age < 50y). The present study investigates whether tumor microbiota may mediate this association in CRC. Materials and methods We enrolled 140 patients with CRC and 30 patients with polyps from the biobank of National Taiwan University Hospital, respectively. The tumor and adjacent normal part in each tumor or polyp were collected. V3-V4 regions of bacterial 16S ribosomal RNA gene were amplified and then amplicons were sequenced on the MiSeq system. Patients' clinicopathological characteristics were also recorded. CIMP status was defined by using a 5-gene panel ( p16, MINT1, MINT2, MINT31 and MLH1 ) and MethyLight assay. Analysis of diversity and LefSe (Linear discriminant analysis Effect Size) of tumor microbiota were performed and the results were compared between each subgroup. Results In total, 121 CRC tumors (CRC-T), 115 adjacent normal (CRC-N) and 26 polyps (polyp-T and polyp-N) passed the quality control (sequencing depth > 5000 reads). Microbiota alpha diversity is significantly lower in CRC compared to that in polyps ( p = 1.3 x 10 -11 ). Microbiota alpha diversity is also reduced in CRC patients with high BMI compared to those with low BMI ( p = 0.042). LefSe analyses revealed specific microbiota enriched in each subgroup, including EOCRC, high BMI, and CIMP-high. The Venn diagram revealed multiple overlapping bacteria taxa between the high BMI and the CIMP-high or EOCRC subgroups. Conclusion We found the diversity of microbiota in CRC with high BMI is significantly lower than that in CRC with low BMI and identified specific bacteria taxa shared between high BMI and CIMP-high or EOCRC tumors. The clinical applications of these bacterial taxa warrant further exploration.

利益披露 Disclosure

K. Chen, None.. J. Hsu, None.. Y. Su, None.. S. Lin, None.. B. Lin, None.. K. Tsai, None.. L. Tseng, None.. J. Tsai, None.. C. Tsai, None.. T. Liu, None.

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