基于邻近性的基因表达谱分析鉴定出血管周围、神经周围及假包膜邻近的人类前列腺癌细胞中的多通路激活

Prostate cancer (PCa) progression is critically driven by the tumor microenvironment (TME), which imposes selective pressures and dictates specialized invasive behaviors, such as perineural and lymphovascular invasion. We hypothesized that heterogeneity within the tumor allows for the selection of functionally specialized cancer cell clones based on their proximity to TME niches. Using Spatial Transcriptomics, we profiled cancer cell populations at three critical invasive fronts: near nerves, vessels, and the prostatic pseudo-capsule from 55 human prostate tissue datasets. Differential expression analysis revealed distinct, niche-specific functional signatures: neurotropic clones near nerves exhibited a loss of prostate identity and a gain of neural adhesion genes (e.g., NCAM1, NTN1) consistent with perineural invasion; perivascular clones showed upregulation of ECM remodeling and an EMT-like state (e.g., ANXA2, CCN1), suggesting priming for intravasation; and pseudo-capsule-adjacent clones displayed a highly proliferative phenotype (e.g., CCND1, CDK4). These results demonstrate that the TME drives the functional specialization of PCa cells, refining our understanding of invasion and nominating several niche-specific pathways as potential therapeutic targets. Studies are currently ongoing to validate these signatures, refine heterogeneity scoring, and cross-link the identified genes with pharmacological databases for future in-vitro inhibition assays.