PO.PR02.03 · 预防研究
市售蓝莓花青素含量的差异及其对膳食癌症预防的意义
Variability in anthocyanin content among commercial blueberries and implications for dietary cancer prevention
作者与单位 Authors & Affiliations
摘要 Abstract
中文摘要
通过膳食进行癌症预防的早期干预可能降低肿瘤风险，实验模型和将饮食与较低癌症发病率相关联的流行病学研究均支持这一观点。许多可食用水果的癌症预防机制由生物活性植物营养素介导。例如，在护士健康研究（Nurses' Health Study）中，每周食用两份蓝莓与乳腺癌患者癌症死亡率降低25%相关；而在一项荟萃分析中，花青素摄入与结直肠癌风险降低22%相关。花青素是蓝莓中一类主要的生物活性类黄酮，在临床前模型中可调节内皮细胞生长并抑制VEGF驱动的血管生成，提示不同蓝莓品种间花青素浓度的差异可能影响其抗癌潜力。商业种植的蓝莓有多种品种和产区，可能导致花青素含量和生物活性存在显著差异。为研究这一点并筛选用于机制研究的候选品种，我们从11个市售来源采集了蓝莓。样本于-20°C冷冻、粉碎、溶剂萃取，并以经过验证的标准品矢车菊素-3-O-葡萄糖苷（C3G）为参照，采用高效液相色谱法（HPLC）进行三次重复分析。花青素浓度范围为0.204至4.244 mg/g湿重（以C3G当量计）。野生矮丛缅因蓝莓的花青素浓度最高，且具有独特的色谱图谱；而在美国商业蓝莓生产中占主导地位的高丛品种则表现出更大的水平差异，其中若干品种排名也相对靠前。总体而言，这些发现表明不同类型蓝莓间花青素含量存在显著差异，并为选择特定品种用于膳食血管生成预防的机制研究提供了依据。基于这些结果，我们选择了具有代表性的提取物（高花青素含量和低花青素含量），用于后续内皮细胞管腔形成的体外研究。此类比较性生化表征对于优化未来研究蓝莓来源花青素癌症预防潜力的实验设计至关重要。
查看英文原文 English abstract
Early intervention through dietary cancer prevention may reduce tumor risk, as supported by experimental models and epidemiologic studies linking diet to lower cancer incidence. Mechanisms of cancer prevention for many edible fruits are mediated by bioactive phytonutrients. For example, consumption of two servings of blueberries per week was associated with a 25% lower cancer mortality among breast cancer patients in the Nurses' Health Study, and anthocyanin consumption was linked to a 22% reduced risk of colorectal cancer in a meta-analysis. Anthocyanins, a major class of bioactive flavonoids in blueberries, modulate endothelial growth and inhibit VEGF-driven angiogenesis in preclinical models, suggesting that differences in anthocyanin concentration across blueberry cultivars may influence their anticancer potential. Commercially grown blueberries are available in a variety of cultivars and regions, potentially resulting in substantial variability in anthocyanin content and bioactivity. To investigate this and identify candidates for mechanistic studies, blueberries were collected from 11 commercially available sources. Samples were frozen at -20°C, pulverized, solvent-extracted, and analyzed by high-performance liquid chromatography (HPLC) in triplicate, using cyanidin-3-0-glucoside (C3G) as a validated standard. Anthocyanin concentrations ranged from 0.204 to 4.244mg/g wet weight (C3G equivalents). Wild lowbush Maine blueberries contained the highest anthocyanin concentrations and had a distinct chromatographic profile, while highbush cultivars, which dominate U.S. commercial blueberry production, showed more variable levels, with several also ranking relatively high. Overall, these findings demonstrate that anthocyanin content differs significantly among blueberry types and provide a rationale for selecting specific cultivars for mechanistic studies of dietary angioprevention. Based on these results, representative extracts (high and low anthocyanin content) were selected for subsequent in vitro studies of endothelial tube formation. Such comparative biochemical characterization is essential to optimize the design of future investigations into the cancer-preventive potential of blueberry-derived anthocyanins.
利益披露 Disclosure
N. Y. Li, None..
L. Firestone, None..
W. W. Li, None..
V. W. Li, None.