PO.TB04.01 · 肿瘤生物学
用于高通量筛选应用的批量癌症球状体保存及解冻后活力
Bulk cancer spheroid preservation and post-thaw viability for high-throughput screening applications
作者与单位 Authors & Affiliations
摘要 Abstract
中文摘要
三维癌症球状体提供了生理学相关的模型，能够密切模拟体内肿瘤结构，使其成为药物筛选和肿瘤生物学研究的宝贵工具。尽管具有这些优势，但其融入高通量工作流程往往受到劳动密集型且时间敏感的培养过程的阻碍。在本研究中，我们采用Corning公司的12K Flask形式，考察了癌症球状体系批量冷冻保存的可行性，随后评估了解冻后活力及在下游检测中的表现。我们的研究结果表明，批量冷冻可有效保存多种癌症细胞类型的球状体形态和细胞活力。解冻后的球状体保留了结构完整性和代谢活性，支持其用于筛选应用。这种可扩展的方法提高了实验的可重复性和工作流程的灵活性，使3D模型在临床前研究中得到更广泛的采用。这些结果支持将冷冻保存的球状体纳入高通量平台，从而推进3D培养系统在癌症药物发现中的应用价值。
查看英文原文 English abstract
Three-dimensional cancer spheroids provide physiologically relevant models that closely mimic in vivo tumor architecture, making them valuable tools for drug screening and tumor biology research. Despite their advantages, integration into high-throughput workflows is often hindered by labor-intensive and time-sensitive culture processes. In this study, we investigated the feasibility of bulk cryopreservation of cancer spheroid lines using Corning's 12K Flask format, followed by assessment of post-thaw viability and performance in downstream assays. Our findings demonstrate that bulk freezing effectively preserves spheroid morphology and cellular viability across multiple cancer cell types. Post-thaw spheroids retained structural integrity and metabolic activity, supporting their use in screening applications. This scalable approach enhances experimental reproducibility and workflow flexibility, enabling broader adoption of 3D models in preclinical research. These results support the incorporation of cryopreserved spheroids into high-throughput platforms, advancing the utility of 3D culture systems in cancer drug discovery.
利益披露 Disclosure
H. Sherman, None.