PO.BCS01.02 · 生物信息与计算

共享的肠道病毒组特征凸显HIV-蠕虫共感染中潜在的早期结直肠癌标志物

Shared gut virome profiles highlight potential early colorectal cancer markers in HIV-helminth co-infection

编号 1414 展板 8 时间 4/20 09:00–12:00 区域 Section 3 主讲 Zodwa Dlamini, PhD

分会场 Application of Bioinformatics to Cancer Biology 2

查看 PDF 下载 PDF 🔒 查看 / 下载完整 PDF 需登录并开通下载套餐 · 查看套餐 / 开通 AACR 官方页面

作者与单位 Authors & Affiliations

Botle Precious Damane¹, Thanyani V. Mulaudzi¹, Jonathan Featherston², Sayed Shakeel Kader³, Pragalathan Naidoo⁴, Zodwa Dlamini⁵, Zilungile Lynette Mkhize-Kwitshana⁶

¹Department of Surgery, Steve Biko Academic Hospital, University of Pretoria, Pretoria, South Africa,²Division of National Health Laboratory Service, The National Institute For Communicable Diseases Of South Africa, Sandringham, South Africa,³Department of Surgery, University of KwaZulu-Natal, Durban 4001, Congella, South Africa, University of KwaZulu Natal, KwaZulu Natal, South Africa,⁴Department of Medical Microbiology, College of Health Sciences, School of Laboratory Medicine & Medi, University of KwaZulu Natal, KwaZulu Natal, South Africa,⁵Pan African Cancer Research Institute (PACRI), University of Pretoria, Pretoria, South Africa,⁶Biomedical Sciences Department; School of Life and Consumer Sciences, College of Agriculture and Env, University of South Africa, Johannesburg, South Africa

摘要 Abstract

中文摘要

背景：在HIV和蠕虫高流行地区，共感染可能以促进致癌过程的方式重塑肠道病毒组。表征这些改变或可在高危人群中识别出早期结直肠癌（CRC）生物标志物。方法：分析来自CRC患者、HIV感染者、蠕虫感染者、HIV-蠕虫共感染个体以及未感染对照的粪便来源宏基因组数据。使用宏基因组共组装流程在科和属水平上对病毒分类群进行定量。通过分层聚类和热图可视化比较各组间的丰度模式。结果：CRC样本形成了独特的聚类，其中Siphoviridae（−1.6 ± 0.41；q = 0.0013）、Podoviridae（2.3 ± 0.83；q = 0.031）、未分类Caudovirales（−1.6 ± 0.51；q = 0.010）、Virus sp. ctWxR2（−14.7 ± 5.04；q = 0.019）以及CrAss样病毒sp. ctt4r3（22.9 ± 5.00；q = 9.9×10⁻⁵）的丰度发生改变。HIV-蠕虫共感染样本形成了一个与CRC重叠的亚聚类，在Siphoviridae（−2.0 ± 0.39；q = 5.3×10⁻⁶）、未分类Caudovirales（−2.6 ± 0.48；q = 4.7×10⁻⁶）、Virus sp. ctWxR2（−21.1 ± 4.79；q = 0.00011）以及CrAss样病毒sp. ctt4r3（24.8 ± 4.78；q = 2.2×10⁻⁷）方面显示出相似的富集。仅HIV样本与对照相似，而仅蠕虫样本则表现出中间型特征。结论：HIV-蠕虫共感染个体显示出与CRC高度一致的特征，表明慢性免疫扰动可能造成类似于早期CRC相关菌群失调的病毒组状态。这些共享的病毒特征可作为在高负担环境中进行CRC风险分层的潜在非侵入性生物标志物。需要进行纵向分析以阐明其时间动态，并识别病毒/噬菌体重构可能通过哪些细菌宿主和功能通路促进CRC的发生。

查看英文原文 English abstract

Background: In regions with high HIV and helminth prevalence, co-infection may reshape the gut virome in ways that promote oncogenic processes. Characterizing these alterations may identify early colorectal cancer (CRC) biomarkers in high-risk populations. Methods: Stool-derived metagenomic data from CRC patients, HIV-infected, helminth-infected, HIV-helminth co-infected individuals, and uninfected controls were analyzed. Viral taxa were quantified at family and genus levels using a metagenomic coassembly pipeline. Hierarchical clustering and heatmap visualisation compared abundance patterns across groups. Results: CRC samples formed distinct clusters with altered abundance of Siphoviridae (−1.6 ± 0.41; q = 0.0013), Podoviridae (2.3 ± 0.83; q = 0.031), unclassified Caudovirales (−1.6 ± 0.51; q = 0.010), Virus sp. ctWxR2 (−14.7 ± 5.04; q = 0.019), and CrAss-like virus sp. ctt4r3 (22.9 ± 5.00; q = 9.9×10⁻⁵). HIV-helminth co-infected samples formed a subcluster overlapping CRC, showing similar enrichments in Siphoviridae (−2.0 ± 0.39; q = 5.3×10⁻⁶), unclassified Caudovirales (−2.6 ± 0.48; q = 4.7×10⁻⁶), Virus sp. ctWxR2 (−21.1 ± 4.79; q = 0.00011), and CrAss-like virus sp. ctt4r3 (24.8 ± 4.78; q = 2.2×10⁻⁷). HIV-only samples resembled controls, while helminth-only samples displayed intermediate profiles. Conclusion: HIV-helminth co-infected individuals showed closely aligned profiles with CRC, indicating that chronic immune perturbation may create virome states resembling early CRC-associated dysbiosis. These shared viral signatures could serve as potential non-invasive biomarkers for CRC risk stratification in high-burden settings. Longitudinal analyses are needed to clarify temporal dynamics and identify the bacterial hosts and functional pathways through which viral/phage restructuring may promote CRC development.

利益披露 Disclosure

B. P. Damane, None.. T. V. Mulaudzi, None.. J. Featherston, None.. S. S. Kader, None.. P. Naidoo, None.. Z. Dlamini, None.. Z. L. Mkhize-Kwitshana, None.

← 返回 AACR 2026 检索