PO.ET02.01 · 实验与分子治疗
基于板的偶联技术用于ADC开发中的抗体与载荷优化
Plate-based conjugation for antibody and payload optimization in ADC development
作者与单位 Authors & Affiliations
摘要 Abstract
中文摘要
ADC通常是逐个合成的，不适合使用平台化方法进行早期筛选。我们开发了一个基于板的偶联平台，利用基于马来酰亚胺的连接子-载荷，将早期筛选与体外表征相整合。凭借标准化、可与检测兼容的方案，该高通量系统能够快速评估DAR、纯度、产率、细胞毒性和结合能力。它支持采用固定连接子-载荷进行抗体筛选、采用固定抗体进行载荷筛选，以及在不同条件下进行ADC表征，为合理化ADC设计提供了强大的工具。
查看英文原文 English abstract
ADCs are typically synthesized one by one and not amenable to early screening using a platform method. We developed a plate-based conjugation platform that integrates early screening with in vitro characterization using maleimide-based linker-payloads. With a standardized, assay-compatible protocol, this high-throughput system rapidly assesses DAR, purity, yield, cytotoxicity, and binding. It supports antibody screening with fixed linker-payloads, payload screening with fixed antibodies, and ADC characterization under varied conditions, providing a powerful tool for rational ADC design.
利益披露 Disclosure
Z. Fereshteh, None..
M. S. Chalasani, None..
R. Sarkar, None..
J. Dugal-Tessier, None..
N. Jain, None.