PO.TB07.02 · 肿瘤生物学
自然杀伤细胞的形态学发现及利用胶束化营养保健品增强其活性
The morphological discovery of the natural killer cells and enhancement of their activity using micellized nutraceuticals
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摘要 Abstract
中文摘要
我们观察到绵羊红细胞以及后来的癌症干细胞（CSC）在无需事先免疫的情况下被循环淋巴细胞自发破坏。这些淋巴细胞随后被称为自然杀伤细胞（NKC），是血液中抵御病毒和癌症干细胞的第一道防线。我们的许多研究使用营养保健品来增强NKC对癌细胞的活性。我们对NKC的最初发现是在仔细观察离心到显微镜载玻片上绵羊红细胞（SRBC）铺层上的未免疫小鼠淋巴细胞时发生的。这些“杀伤”淋巴细胞在37℃孵育15分钟内接触即可完全破坏周围的外源红细胞，在SRBC铺层中形成清晰的空斑。我们能够使用正常小鼠脾细胞的连续等渗牛血清白蛋白梯度（密度=1.0498）将这些杀伤细胞纯化至少5倍。经戊二醛固定和吉姆萨染色后，Nomarski显微镜显示杀伤细胞为小至中等淋巴细胞。扫描电子显微镜揭示这些细胞表面粗糙。随后，使用K562白血病干细胞铺层作为淋巴细胞的靶细胞。我们观察到其与癌细胞结合，随后破坏其细胞膜，如Nomarski显微镜所示。K562膜的“出泡”破坏在透射和扫描电子显微镜图像中得以可视化。有趣的是，NKC表现出类似獠牙的伪足膜，使其能够将毒液注入癌细胞。此外，可以观察到粗面内质网在破坏发生的一侧排列，表明细胞溶解蛋白的产生。我们已投入大量研究以鉴定增强NKC和整体免疫的营养保健品，重点是通过胶束化制剂提高生物利用度，相关内容将进行展示。https://youtu.be/HNP1EAYLhOs?si=QmsD3xDo5C9FrRZw
查看英文原文 English abstract
We observed that sheep erythrocytes and, later, Cancer Stem Cells (CSCs) are spontaneously destroyed by circulating lymphocytes without prior immunization. These lymphocytes, subsequently referred to as Natural Killer Cells (NKC), are the first line of defense in the blood against viruses and cancer stem cells. Much of our research has used nutraceuticals to enhance NKC activity against cancer cells. Our initial discovery of the NKC occurred while carefully observing non-immunized mouse lymphocytes centrifuged onto a lawn of sheep Red Blood Cells (SRBC) on a microscope slide. These “killer” lymphocytes would completely destroy the surrounding foreign erythrocytes on contact within a 15-minute incubation at 37 °C, forming clear plaques in a lawn of SRBC. We were able to purify these killer cells at least fivefold using continuous isotonic bovine serum albumin gradients (density = 1.0498) of normal mouse spleen cells. After glutaraldehyde fixation and Giemsa staining, Nomarski microscopy showed that the killer cells were small to medium lymphocytes. Scanning electron microscopy revealed a rough surface for these cells. Subsequently, a lawn of K562 leukemic stem cells was used as a target for the lymphocytes. We observed binding to the cancer cells, followed by the destruction of their cell membranes, as shown by Nomarski microscopy. The “blebbing” destruction of the K562 membranes is visualized in the transmission and scanning electron microscopy images. Interestingly, the NKCs exhibit pseudopodia membranes that resemble fangs, allowing them to deposit their venom into the cancer cell. Additionally, one can observe the rough endoplasmic reticulum aligning itself on the side where destruction occurs, indicating the production of cytolytic proteins. We have devoted significant research to identifying nutraceuticals that enhance NKCs and overall immunity, with an emphasis on increasing bioavailability through micellized formulations, which will be presented.https://youtu.be/HNP1EAYLhOs?si=QmsD3xDo5C9FrRZw
利益披露 Disclosure
J. T. Thornthwaite, None..
D. Strasser, None..
K. A. Thornthwaite, None.