PO.BCS01.10 · 生物信息与计算
模拟通过巨胞饮作用的跨内皮纳米颗粒转运
Simulating trans-endothelial nanoparticle transport via macropinocytosis
作者与单位 Authors & Affiliations
摘要 Abstract
中文摘要
巨胞饮作用是纳米颗粒跨内皮转运进入实体瘤的关键机制。它利用细胞膜皱褶来捕获循环中的纳米颗粒。内皮细胞皱褶无法在体内被独立且系统地改变，从而阻碍了参数特异性分析。我们开发了一个计算机模拟模型来考察巨胞饮作用对纳米颗粒的捕获。我们发现，纳米颗粒的捕获率随皱褶数量以及皱褶更新时间陡峭上升，直至饱和。根据我们的数据，我们推导出一个描述巨胞饮作用捕获纳米颗粒的方程。这一关系揭示了皱褶动力学如何决定纳米颗粒捕获效率。
查看英文原文 English abstract
Macropinocytosis is a key mechanism in transendothelial nanoparticle transport into solid tumours. It uses cell membrane ruffles to capture circulating nanoparticles. Endothelial cell ruffles cannot be independently and systematically varied in vivo , preventing parameter-specific analysis. We developed an in silico model that examines nanoparticle capture by macropinocytosis. We discover that nanoparticle capture rates rise steeply until saturating with ruffle number as well as ruffle turnover time. From our data we derive an equation describing the nanoparticle capture by macropinocytosis. This relationship reveals how ruffle dynamics govern nanoparticle capture efficiency.
利益披露 Disclosure
M. Skiba, None.
H. T. Han,
Neogene Therapeutics Employment.
W. Chan, None.