NEU-002的转化开发：工程化治疗性弹性蛋白酶通过腹腔内和静脉给药展现出全身性抗肿瘤活性

NEU-002 is a systemically deliverable engineered elastase targeting the ELANE pathway and is under development for solid tumors. To advance NEU-002 toward clinical translation, two optimized candidates, N17474 and N17482, were generated through rational sequence engineering. This optimization strategy focused on preserving elastase activity in blood by protection from serine protease inhibitors, as evaluated using a range of biochemical assays. Furthermore, we show that NEU-002 program compounds maintained robust anti-tumor efficacy in the CT26 colon cancer model following intravenous administration, demonstrating that essential functional properties were retained during optimization. In addition, we explored a new intraperitoneal route of administration to expand delivery options for NEU-002 and observed reductions in tumor burden in orthotopic colon cancer mouse models, supporting the feasibility of accessing tumors within visceral compartments. Collectively, these findings establish a strong translational foundation for NEU-002 and, together with planned pharmacokinetic studies in non-human primates, will directly inform the selection of the final development candidate for advancement toward clinical evaluation in solid tumor indications. Generative artificial intelligence was used to assist in drafting text for this abstract.