PO.CL05.09 · 临床研究
聚苯乙烯微塑料对人膀胱癌细胞致癌效应的研究
Investigation of carcinogenic effects of polystyrene microplastic on human bladder cancer cells
该海报暂无可下载的资料
AACR 官方页面
作者与单位 Authors & Affiliations
摘要 Abstract
中文摘要
塑料污染已成为全球性环境问题。人类通过摄入、吸入和皮肤接触，越来越多地暴露于微塑料（MP）和纳米塑料（NP）。这些颗粒可能通过引发炎症、改变免疫反应和破坏尿路上皮屏障来影响脑、肝、肾和膀胱等器官——尿路上皮屏障破坏是膀胱癌和间质性膀胱炎的一个既定风险因素。我们研究了人膀胱癌细胞系（SW-78和UMUC-3）对微（0.5 μm）和纳米（0.03 μm）聚苯乙烯微塑料的摄取。两种细胞系与对照相比均显示出增殖改变。SW-78细胞对8,000、16,000和32,000 p/mL有显著反应，而64,000 p/mL对细胞增殖有毒性。在UMUC-3细胞中，增殖在所有浓度下均以时间依赖性方式增加。此外，与对照相比，UMUC-3细胞的迁移未受0.03 μm PS颗粒的显著影响，而0.5 μm PS颗粒则影响迁移。在SW-78细胞中，迁移被较低浓度的0.03 μm颗粒显著诱导，但在64,000 p/mL时显著降低。我们还证实，较高剂量的塑料颗粒以浓度依赖性方式引起显著的DNA损伤和ROS产生。在活细胞成像中，PS颗粒显示出MP与细胞表面的强附着，而共聚焦成像证实了PS颗粒的摄取、胞质积累和核转位。我们的发现提示，PS微塑料以剂量和大小依赖性方式影响膀胱癌细胞的增殖和迁移，与癌症进展所涉及的机制一致。胞质中微塑料的存在很可能破坏细胞代谢过程，值得进一步研究。鉴于塑料污染的加剧，微塑料可能代表膀胱癌的新兴风险因素，突显了进行全面的毒理学和流行病学研究的必要性。
查看英文原文 English abstract
Plastic pollution has become a global environmental problem. Humans are increasingly exposed to microplastics (MPs) and nano-plastics (NPs) through ingestion, inhalation, and skin contact. These particles may affect organs such as the brain, liver, kidneys, and bladder by triggering inflammation, altering immune responses, and disrupting the urothelial barrier-an established risk factor for bladder cancer and interstitial cystitis. We investigated the uptake of micro (0.5 µm) and nano-plastic (0.03 µm) polystyrene microplastics by human bladder cancer cell lines (SW-78 and UMUC-3). Both cell lines showed altered proliferation compared with controls. SW-78 cells responded significantly to 8,000, 16,000, and 32,000 p/mL, while 64,000 p/mL was toxic to cell proliferation. In UMUC-3 cells, proliferation increased at all concentrations in a time-dependent manner. Moreover, cell migration in UMUC-3 cells was not significantly influenced by 0.03 µm PS particles compared with controls, whereas 0.5 µm PS particles affected migration. In SW-78 cells, migration was significantly induced by lower concentrations of 0.03 µm particle but was significantly reduced at 64,000 p/mL. We also confirmed that higher doses of plastic particles caused significant DNA damage and ROS production in a concentration-dependent manner. PS particles showed strong attachment of MPs to the cell surface in live-cell imaging, while confocal imaging confirmed uptake, cytoplasmic accumulation, and nuclear translocation of the PS particles. Our findings suggest that PS microplastics influence bladder cancer cell proliferation and migration in a dose- and size-dependent manner, consistent with mechanisms involved in cancer progression. The presence of microplastics in the cytoplasm is likely to disrupt cellular metabolic processes, warranting further investigation. Given the rise in plastic pollution, microplastics may represent emerging risk factors for bladder cancer, underscoring the need for thorough toxicological and epidemiological studies.
利益披露 Disclosure
S. Ram, None.