用于从细胞和组织进行全面多组学分析的自动化顺序蛋白质/DNA/RNA提取工作流程

Introduction: The integration of proteomics, genomics, and transcriptomics is crucial for comprehensive biological insights. We have developed an automated sequential Protein/DNA/RNA workflow designed to reproducibly isolate high-quality protein, DNA, and RNA from the same sample using the KingFisher™ SamplePrep Purification System, enhancing the efficiency, through-put and depth of multiomics studies. Methods: Our sequential Protein/DNA/RNA extraction workflow utilizes a magnetic bead-based approach, leveraging the well renowned Dynabeads™ and MagMAX™ magnetic beads technologies. This enables efficient isolation of protein, DNA, and RNA from as few as 1,000 cells or 1 mg of tissue. The workflow is compatible with both manual and automated processing on the KingFisher™ sample purification system, accommodating a wide range of sample numbers from low to high throughput. Results: Mass Spectrometry (MS): The workflow produces high-quality proteins suitable for MS analysis. Comparative studies with the EasyPep™ MS Sample Prep Kit show similar chromatograms, peptide properties, and distribution profiles among biological processes and cellular components, as well as absence of incompatible reagents. Next-Generation Sequencing (NGS): The workflow yields high-quality nucleic acids compatible with NGS. Whole genome sequencing and rRNA depletion sequencing demonstrate excellent molecular depth, coverage and uniformity, with robust QC metrics across various sample types. Conclusion: The sequential Protein/DNA/RNA workflow offers a versatile and efficient solution for multiomics research, providing high-quality analytes compatible with both MS and NGS. This magnetic bead-based workflow, utilizing Dynabeads™ and MagMAX™ magnetic beads, is ideal for precious and small samples, enabling reproducible data and high throughput capabilities. For Research Use Only. Not for use in diagnostic procedures.