A modular positioning platform for standardized ex vivo specimen analysis in preclinical optical imaging

Efficient and organized handling of ex vivo specimens is critical for advancing preclinical imaging workflows, particularly in studies requiring high-throughput analysis of tumors, organs, and bone tissues. We developed a novel modular ex vivo sample holder, specifically designed to integrate seamlessly with Revvity's IVIS optical imaging systems for bioluminescence, fluorescence, or x-ray imaging applications. This innovative accessory addresses key challenges in specimen organization, imaging throughput, and experimental reproducibility. In this study, we designed two ex-vivo sample holders with 16-25 separate non-reflective chambers that enable researchers to image multiple samples simultaneously and efficiently. Each chamber has light-blocking walls to prevent crosstalk of signal emission and sample. A wide range of ex vivo specimens including brain, heart, lung, kidneys, liver, spleen, bladder, and gastrointestinal tract were placed on the sample holder with a unique optically dark sheet and imaged using Revvity's IVIS optical systems. We tested multiple types of black sheets and the ideal optically dark sheet was selected based on minimum background effects, reproducibility, and ease-of-operation. The sample holder is designed to incorporate the disposable optically dark paper and minimizes cross-contamination of ex-vivo samples. This paper provides a clean, non-reflective surface that is easily replaceable between experiments, ensuring optimal imaging conditions. Furthermore, the holder's design enhances workflow efficiency by reducing setup time and facilitating rapid specimen arrangement, making it ideal for high-throughput imaging studies. Additionally, IVIS software tools support region of interest (ROI) template creation, allowing for precise and consistent quantitative analysis. The holder's modular compartments allow for different configurations that can be customized to accommodate varying specimen sizes, offering flexibility across diverse experimental needs. This accessory represents a significant advancement for ex vivo optical imaging, providing researchers with a practical solution for improving imaging efficiency, reproducibility, and data quality. By combining ergonomic design, modularity, and software integration, the novel ex vivo sample holder empowers laboratories to achieve greater throughput and precision in preclinical imaging applications.