Plate-based conjugation for antibody and payload optimization in ADC development

ADCs are typically synthesized one by one and not amenable to early screening using a platform method. We developed a plate-based conjugation platform that integrates early screening with in vitro characterization using maleimide-based linker-payloads. With a standardized, assay-compatible protocol, this high-throughput system rapidly assesses DAR, purity, yield, cytotoxicity, and binding. It supports antibody screening with fixed linker-payloads, payload screening with fixed antibodies, and ADC characterization under varied conditions, providing a powerful tool for rational ADC design.