Novel mouse models for cancer research

The Jackson Laboratory (JAX) is a centralized resource of genetically defined mutant and transgenic mice to support the scientific research community. This poster will highlight new models available for specific cancers, xenograft recipients, immunodeficient platforms for PDX studies and multi-purpose tool strains having CRISPR utility, conditional/inducible expression (Cre-lox, FLP-frt, Tet-On/-Off), optogenetic function and calcium-sensing technologies. The NSG (NOD scid IL2rgammac-/-) portfolio of strains for transplantation/engraftment and humanized mouse research continues to grow, with recent humanized alleles ( e.g. , IL6, TSLP) expanding the options for immune-oncology studies. New mouse lines for cancer immunology and antibody therapeutic studies combine humanized FCGRT and ALB alleles for human preclinical pharmacokinetics (HuPK), as well as humanized PD-1 and PD-L1 alleles. Novel fluorescent strains include Gene Editing Reporters for visualizing homology-directed repair (HDR) and non-homologous end joining (NHEJ) repair events, as well as several CRISPR Editing, Bxb1 Integrase Editing, Base Editor, Prime Editor and DNA repair pathway tools. In addition to cryopreserving each strain, JAX employs a genetic quality control program that confirms mutation identity and genetic background, and screens for common unwanted alleles (via testing for neo, Cre, FLP, GFP, RFP, etc.). Cancer researchers are encouraged to query the collection of innovative mouse models using the JAX Mouse Search website ( mice.jax.org ). This resource includes models created by many generous donating institutions. Researchers are encouraged to donate their mouse lines via a very short Strain Submission form ( jax.org/donate-a-mouse ). Please also visit the JAX Oncology webpage for cancer research related strains and resources ( jax.org/jax-mice-and-services/solutions-by-therapeutic-area/oncology ). JAX is supported by the NIH, The Howard Hughes Medical Institute and other private charitable foundations.